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Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by?Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the?pac?gene.
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 μg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/mL. Gibco? Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8) in 10 vials each containing 1 mL.
Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.
See the complete list, or find products for:
??Contamination control
??Eukaryotic and bacterial selection
See recommendations for?working concentrations for selection antibiotics.
Learn more about the?use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
