Use?this?kit?to?isolate?pure?and?viable?untouched?T-cells?from?PBMC?by?negative?isolation.?The?kit?depletes?B?cells,?NK?cells,?monocytes,?platelets,?dendritic?cells,?granulocytes?and?erythrocytes.?The?negatively?isolated?human?T-cells?are?left?in?the?sample?and?have?not?been?in?contact?with?the?Dynabeads.?The?bead?and?antibody-free?T-cells?are?truly?untouched,?and?in?perfect?shape?for?any?functional?assay?and?application.

??High?purity,?recovery?and?viability?of?human?untouched?T?cells
??Easy?to?use?and?to?scale?up
??No?columns?required
Methodology:
An?antibody?mix?towards?the?non-T?cells?is?added?to?the?sample?and?allowed?to?bind?to?the?cells.?Dynabeads??are?added?and?will?bind?to?the?antibody-labeled?cells?during?a?short?incubation.?The?bead-bound?cells?are?quickly?separated?on?a?magnet?and?discarded.?The?remaining?negatively?isolated?and?untouched?human?T-cells?can?be?directly?analyzed?in?a?flow?cytometer?and?used?in?any?application.

Note:?A?negative?isolation?strategy?isolates?pure?and?truly?untouched?cells.?When?maximum?cell?purity?is?critical,?a?positive?cell?isolation?strategy?is?recommended

Starting?Sample:
PBMC
For?Research?Use?Only.?Not?for?use?in?diagnostic?procedures.

Achieve?functional?knockout?in?human?primary?T-cells