


| Positive WB detected in | mouse thymus tissue, rat spleen tissue, human lymph node tissue |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
| Positive FC detected in | PMA and ionomycin treated MOLT-4 cells |
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite?488 and CoraLite?647 conjugated versions of this antibody.
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |

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