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產品信息
| 名稱 | KYSE-150 (人食管鱗癌細胞) (STR鑒定正確) |
| 別稱 | KYSE 150; KYSE150; Kyse150; KY150 |
| 背景描述 | Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice. |
| 種屬 | 人 |
| 年齡（性別） | 女；49歲 |
| 組織來源 | 食管 |
| 生長特性 | 貼壁細胞 |
| 細胞形態 | 上皮細胞樣 |
| 細胞類型 | 腫瘤細胞 |
| 腫瘤類型 | 食管癌細胞 |
| 生物安全等級 | 1 |
| 生長培養基 | RPMI-1640＋Ham's F-12＋10% FBS＋1%P/S |
| 推薦傳代比例 | 1:4-1:8 |
| 推薦換液頻率 | 2~3次/周 |
| 倍增時間 | 13.7 hours (PubMed=1728357); 23 hours (PubMed=25984343); <25 hours, less when serum amount is increased (DSMZ) |
| 凍存條件 | 凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮 |
| 培養條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ |
| 致瘤性 | Yes, in nude mice |
| 保藏機構 | DSMZ; ACC-375 |
STR位點信息
| Amelogenin | X |
| CSF1PO | 12,13 |
| D2S1338 | 25 |
| D3S1358 | 15,16 |
| D5S818 | 12,13 |
| D7S820 | 10,11 |
| D8S1179 | 10,15 |
| D13S317 | 8,11 |
| D16S539 | 9,11 |
| D18S51 | 14 |
| D19S433 | 15,15.2 |
| D21S11 | 30,31 |
| FGA | 21,24 |
| PentaD | 10 |
| PentaE | 12,18 |
| TH01 | 7,9 |
| TPOX | 8 |
| vWA | 16,17 |
| D6S1043 | 18,20 |
| D12S391 | 19,22 |
| D2S441 | 10,11 |
