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產品信息
| 名稱 | M-07e (人巨細胞白血病細胞) (STR鑒定正確) |
| 別稱 | M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E |
| 背景描述 | Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available |
| 種屬 | 人類 |
| 年齡（性別） | 女性，6個月 |
| 組織來源 | 外周血 |
| 生長特性 | 懸浮細胞 |
| 細胞形態 | 淋巴母細胞樣 |
| 細胞類型 | 腫瘤細胞 |
| 腫瘤類型 | 白血病細胞 |
| 生物安全等級 | 1 |
| 生長培養基 | RPMI-1640＋10% FBS＋8ng/mL GM-CSF+1% P/S |
| 推薦傳代比例 | 5×10^5-1×10^6cells/mL |
| 推薦換液頻率 | 2~3次/周 |
| 倍增時間 | ~40小時 |
| 凍存條件 | 凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮 |
| 培養條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ |
| 抗原表達情況 | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR - |
| 注意事項 | 該細胞為懸浮細胞，請注意離心收集細胞懸液；請勿直接倒掉細胞培養液。 |
| 保藏機構 | DSMZ; ACC-104 |
STR位點信息
| Amelogenin | X |
| CSF1PO | 9,10 |
| D2S1338 | 19,25 |
| D3S1358 | 16,19 |
| D5S818 | 11 |
| D7S820 | 11 |
| D8S1179 | 13,16 |
| D13S317 | 10,11 |
| D16S539 | 11 |
| D18S51 | 15,20 |
| D19S433 | 14,15 |
| D21S11 | 30,32.2 |
| FGA | 18,22 |
| PentaD | 9 |
| PentaE | 12,14 |
| TH01 | 6,8 |
| TPOX | 8 |
| vWA | 16,18 |
| D6S1043 | 13,19 |
| D12S391 | 23,24 |
| D2S441 | 14 |
