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產(chǎn)品信息
| 名稱 | SU-DHL-4 (人B淋巴瘤細(xì)胞) (STR鑒定正確) |
| 別稱 | SUDHL4; Sudhl4; SUDHL-4; Sudhl-4; SuDHL 4; SUD-4; SUD4; SU4; Stanford University-Diffuse Histiocytic Lymphoma-4; DHL-4; DHL4 |
| 背景描述 | Established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| 種屬 | 人類 |
| 年齡（性別） | 男性，38歲 |
| 組織來(lái)源 | 腹腔積液 |
| 生長(zhǎng)特性 | 懸浮細(xì)胞 |
| 細(xì)胞形態(tài) | 淋巴母細(xì)胞樣 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 淋巴瘤細(xì)胞 |
| 生長(zhǎng)培養(yǎng)基 | RPMI-1640＋10% FBS＋1% P/S |
| 推薦傳代比例 | 5×10^5-1×10^6cells/mL |
| 推薦換液頻率 | 2~3次/周 |
| 倍增時(shí)間 | ~40小時(shí) |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度：液氮 |
| 培養(yǎng)條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ |
| 基因表達(dá)情況 | IgG+; Kappa+; IgM-; IgA-; IgD-; Lambda-; This cell line has relatively high expression levels of Bax; Bak; AIF; high caspase-9 activity. |
| 注意事項(xiàng) | 該細(xì)胞為懸浮細(xì)胞，請(qǐng)注意離心收集細(xì)胞懸液；請(qǐng)勿直接倒掉細(xì)胞培養(yǎng)液。 |
| 保藏機(jī)構(gòu) | ATCC; CRL-2957 DSMZ; ACC-495 |
STR位點(diǎn)信息
| Amelogenin | X,Y |
| CSF1PO | 12 |
| D2S1338 | 17,19 |
| D3S1358 | 15,16 |
| D5S818 | 11,12 |
| D7S820 | 8,11 |
| D8S1179 | 13 |
| D13S317 | 12 |
| D16S539 | 11,13 |
| D18S51 | 14,16 |
| D19S433 | 14,15.2 |
| D21S11 | 29,31 |
| FGA | 19,21 |
| PentaD | 9,14 |
| PentaE | 7,12 |
| TH01 | 6,9.3 |
| TPOX | 9,11 |
| vWA | 18,19 |
| D6S1043 | 11 |
| D12S391 | 18,20 |
| D2S441 | 11,14 |
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