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產品信息
| 名稱 | NCI-H146 [H146] (人小細胞肺癌細胞) (STR鑒定正確) |
| 別稱 | H146; H-146; NCIH146 |
| 背景描述 | Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected. |
| 種屬 | 人類 |
| 年齡（性別） | 男性，59歲 |
| 組織來源 | 肺 |
| 生長特性 | 懸浮細胞 |
| 細胞形態 | 淋巴母細胞樣 |
| 細胞類型 | 腫瘤細胞 |
| 腫瘤類型 | 肺癌細胞 |
| 生長培養基 | RPMI-1640＋10% FBS＋1% P/S |
| 推薦傳代比例 | 1:2-1:3 |
| 推薦換液頻率 | 2~3次/周 |
| 凍存條件 | 凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮 |
| 培養條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ |
| 基因表達情況 | vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes - |
| 注意事項 | 該細胞為懸浮細胞，請注意離心收集細胞懸液；請勿直接倒掉細胞培養液。 |
| 保藏機構 | ATCC; HTB-173 |
STR位點信息
| Amelogenin | X |
| CSF1PO | 11 |
| D2S1338 | 19 |
| D3S1358 | 11 |
| D5S818 | 12 |
| D7S820 | 9,10 |
| D8S1179 | 12,14 |
| D13S317 | 12 |
| D16S539 | 11 |
| D18S51 | 15,17 |
| D19S433 | 13,14 |
| D21S11 | 30 |
| FGA | 23 |
| PentaD | 13 |
| PentaE | 12 |
| TH01 | 6,9.3 |
| TPOX | 8,11 |
| vWA | 14,16 |
| D6S1043 | 11,19 |
| D12S391 | 18.3,25 |
| D2S441 | 10,14 |
